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Combined chemoseparation and immunoseparation of clonogenic T lymphoma cells from human bone marrow using 2'-deoxycoformycin, deoxyadenosine, 3A1 monoclonal antibody, and complement

Haleem, A.; Kurtzberg, J.; Olsen, G.A.; Rhinehardt-Clark, A.; Leslie, D.S.; Ray, L.; Smith, C.A.; Peters, W.P.; Haynes, B.F.; Bast, R.C.

Cancer Research 47(17): 4608-4612

1987

ISSN/ISBN: 0008-5472
PMID: 3113721
Document Number: 289947
Chemoseparation and immunoseparation techniques have been combined to eliminate malignant clonogenic T lymphoma cells from human bone marrow. Incubation with 5 microM 2'-deoxycoformycin and 500 microM deoxyadenosine has eliminated 2 logs of HSB-2 T lymphoma cells from a 20-fold excess of irradiated human bone marrow. Multiple incubations with 3A1 antibody and rabbit complement eliminated approximately 2 logs of HSB-2 cells from similar mixtures. Used in combination, the 2 techniques eliminated up to 4 logs of T lymphoma cells. Incubation of normal human bone marrow under similar conditions failed to affect growth of granulocyte-macrophage colony-forming cell units, burst-forming erythroid units, or multipotential erythroid-granulocyte-megakaryocyte-macrophage colony-forming hematopoietic progenitor cells units.

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Saarinen, U.M.; Coccia, P.F.; Gerson, S.L.; Pelley, R.; Cheung, N.K. 1985: Eradication of neuroblastoma cells in vitro by monoclonal antibody and human complement: method for purging autologous bone marrow Cancer Research 45(11 Pt 2): 5969-5975
Ruers, T.J.; Buurman, W.A.; van der Linden, C.J. 1987: 2'Deoxycoformycin and deoxyadenosine affect IL 2 production and IL 2 receptor expression of human T cells Journal of Immunology 138(1): 116-122
Irlé, C.; Kaestli, M.; Chapuis, B.; Jeannet, M. 1985: Quantitative determination of human bone marrow T cells following in vitro depletion by Campath-1 monoclonal antibody Schweizerische Medizinische Wochenschrift 115(43): 1517-1518
Kvalheim, G.; Fodstad, O.; Pihl, A.; Nustad, K.; Pharo, A.; Ugelstad, J.; Funderud, S. 1987: Elimination of B-lymphoma cells from human bone marrow: model experiments using monodisperse magnetic particles coated with primary monoclonal antibodies Cancer Research 47(3): 846-851
Alonso, K.; Medenica, R.; Morehead, G. 1994: Long-term culture of autologous bone marrow for marrow reconstitution following high-dose chemotherapy. Purging with tumor surface antigen directed human-human monoclonal antibody Progress in Clinical and Biological Research 389: 111-118
Bai, Y. 1989: Monoclonal antibody in the detection of neuroblastoma cells in bone marrow aspirates Zhonghua Zhong Liu Za Zhi 11(4): 260-262
Berthold, F.; Schumacher, R.; Schneider, A.; Bosslet, K.; Reiter, C. 1989: Removal of neuroblastoma cells from bone marrow by a direct monoclonal antibody rosetting technique Bone Marrow Transplantation 4(3): 273-278
Duerst, R.E.; Ryan, D.H.; Frantz, C.N. 1986: Variables affecting the killing of cultured human neuroblastoma cells with monoclonal antibody and complement Cancer Research 46(7): 3420-3425
Mouchiroud, G.; Berthier, R.; Newton, I.A.; Chapel, A.; Hollard, D. 1985: Monoclonal antibodies against human hemopoietic cells and the separation of progenitor cells from bone marrow Experimental Hematology 13(6): 566-573
Khaitov, R.M.; Petrov, R.V.; Idorovich, I.G.; Ataullakhanov, R.I. 1978: Regulatory role of bone marrow in immunogenesis. II. Analysis of various mechanisms of antibody genesis suppression by the bone marrow B cells in vitro Zhurnal Mikrobiologii Epidemiologii i Immunobiologii 6: 18-22
Kramer, B.; Makrynikola, V.; Bradstock, K. 1992: Effects of mafosfamide on the clonogenic cells in precursor B acute lymphoblastic leukemia: significance for ex vivo purging of bone marrow Progress in Clinical and Biological Research 377: 41-47
Perrot, J.Y.; Boucheix, C.; Mirshahi, M.; Kazatchkine, M.; Bariety, J. 1984: Monoclonal antibodies against surface antigens of lymphoblasts and blood cells or bone marrow recognize constituents of the human nephron Nephrologie 5(2): 53-57
Myklebust, A.T.; Godal, A.; Juell, S.; Pharo, A.; Fodstad, O. 1994: Comparison of two antibody-based methods for elimination of breast cancer cells from human bone marrow Cancer Research 54(1): 209-214
Kharlamova, L.A.; Kolesnikov, A.I.; Vorob'ev, A.I. 1975: Changes in the quantity and level of bone marrow cells and precursor cells of granulopoiesis in organ cultures of human bone marrow Problemy Gematologii i Perelivaniia Krovi 20(12): 45-49
Hantaweepant, C.; Chinthammitr, Y.; Khuhapinant, A.; Sukpanichnant, S. 2016: Clinical Significance of Bone Marrow Involvement as Confirmed by Bone Marrow Aspiration vs. Bone Marrow Biopsy in Diffuse Large B-cell Lymphoma Journal of the Medical Association of Thailand 99(3): 262-269
Hassan, H.T.; Veit, A.; Maurer, H.R. 1991: Recombinant human GM-CSF significantly enhances the cytostatic activity of cell cycle specific anti-leukaemic drugs in HL-60 human myeloid leukaemia clonogenic cells in a capillary clonogenic micro-assay Anticancer Research 11(1): 481-485
Hasthorpe, S.; Rogerson, J.; Green, S.L.; Radley, J.M. 1990: Use of bone marrow somatic cell hybrid lines to generate monoclonal antibodies specifically reactive with rare marrow cells Experimental Hematology 18(3): 223-227
Matsumoto, S.S.; Yu, J.; Yu, A.L. 1983: Inhibition of RNA synthesis by deoxyadenosine plus deoxycoformycin in resting lymphocytes Journal of Immunology 131(6): 2762-2766
Manor, D.; Nussinovich, R.; Yeheskel, A.; Kaempfer, R. 1990: A constitutive antibody in normal human serum directed against rabbit bone marrow cells: lack in parturients, neonates, and hematologic disorders Journal of Laboratory and Clinical Medicine 116(6): 771-778
Lardon, F.; Snoeck, H.W.; Haenen, L.; Lenjou, M.; Nijs, G.; Weekx, S.F.; Van Ranst, P.C.; Berneman, Z.N.; Van Bockstaele, D.R. 1996: The combined effects of all-trans retinoic acid and TGF-beta on the initial proliferation of normal human bone marrow progenitor cells Leukemia 10(12): 1937-1943