The role of isoacceptor transfer RNAs in regulation of age-related changes in the rate of protein synthesis
Khasigov, P.Z.; Nikolaev AYa
Biochemistry International 15(6): 1159-1169
1987
ISSN/ISBN: 0158-5231 PMID: 3440023 Document Number: 289367
In cell-free protein-synthesizing systems containing an S30 extract from liver and brain cortex tissues of 22-day-old fetuses and of male WAG rats (1-900 days old), the minimal rate of protein synthesis was observed in the fetuses, while the maximal one.sbd.in 7-day-old animals. The difference in the rates of protein synthesis correlated with the minimal concentration of total tRNA in the former group and with its maximal concentration in the latter. In fetal tissues, an addition to cell-free systems of total tRNA isolated from homologous tissues of 7-day-old animals augmented protein synthesis up to a level observed in 7-day-old animals, whereas in the tissues of animals belonging to other age groups total tRNA had a far less pronounced stimulating effect which decreased with age. Fractionational of total tRNA and analysis of effects of individual tRNAs on protein synthesis demonstrated that the stimulating influence was induced by tRNA2Arg, tRNA4Arg and tRNA2Val from brain cortex and by tRNA2Leu, tRNA5Leu, tRNA2Val, tRNA1Met and tRNA2Met from liver.