Simple method for observing migrating leucocytes in the skin of mirror carp (Cyprinus carpio L.)
Sövényi, J.F.; Boros, G.
Acta Veterinaria Hungarica 34(1-2): 61-65
1986
ISSN/ISBN: 0236-6290 PMID: 2953193 Document Number: 286174
A special adaptation of Rebuck's skin window for the mirror carp (Cyprinus carpio L.) was developed. The method is based on insertion of a piece of a thin glass square (coverslip) intradermally. During an incubation of less than 24 h, migrating leucocytes attach to the glass square, where they can be stained for observation. It was demonstrated with Mycobacterium phlei bacteria that the glass squares can also be used for locally introducing substances which influence the activity of migrating leucocytes.