Effect of mitomycin C on energy metabolism: essential role of aerobic glycolysis in proliferation of L cells
Ohsaka, N.; Yonezu, T.; Hosokawa, K.; Yamaguchi, H.
Cellular and Molecular Biology 32(4): 477-485
1986
ISSN/ISBN: 0145-5680 PMID: 3091248 Document Number: 285940
Changes in energy metabolism in L cells on treatment with different concentrations of mitomycin C (2-50 ng/ml) for relatively long periods (24 and 48 hr) were studied. These treatments inhibited cell proliferation and DNA synthesis strongly, RNA synthesis less strongly and protein synthesis only slightly. Because of these different effects, the cell volume increased with increase in the dose of antibiotic and the time of treatment. The rate of 14CO2 evolution per unit cell volume from [U-14C]glucose was not altered by any concentration of mitomycin C, whereas the rate of lactate production per unit cell volume increased two to threefold during the first 24 hr of treatment and then decreased greatly between 24 and 48 hr when the concentration of antibiotic was high. The rate of ATP production, calculated from the amounts of 14CO2 and lactate produced, did not change when expressed per unit cell volume, but increased when expressed per cell per doubling time, since the cell volume and the doubling time increased. The ATP content per unit cell volume increased slightly. Thus, the calculated turnover time of ATP was longer with a higher dose of antibiotic. These results reveal a close correlation of aerobic glycolysis with synthesis of macromolecules, and especially DNA.