Rapid drug haptenization procedure: application to gentamicin and quinidine
Regan, C.M.
Journal of Pharmacy and Pharmacology 38(11): 834-836
1986
ISSN/ISBN: 0022-3573 PMID: 2879014 Document Number: 285925
A rapid and simple drug conjugation procedure which utilizes the heterobifunctional reagent N-hydroxysuccinimidyl 6-(4'-azido-2'-nitrophenylamino)hexanoate is described. With this protocol two drugs of diverse structure and solubility have been successfully haptenized. Rabbits immunized with these conjugates yielded drug-specific antibodies as judged by a solid phase radioimmunoassay. Furthermore, using this technique, no cross-reactivity could be detected. Such haptens also elicited an immune response in mice thus further extending their potential for the production of drug specific monoclonal antibodies.