Identification of calmodulin in new-born rat calvaria
Tojyo, Y.; Matsumoto, Y.
Biochemistry International 13(1): 155-161
1986
ISSN/ISBN: 0158-5231 PMID: 3753505 Document Number: 285404
The extract of new-born rat calvaria was chromatographed on DEAE-cellulose. Calmodulin activity was eluted at 0.3 and 0.4 M NaCl and markedly inhibited by trifluoperazine and W-7, calmodulin antagonists. The fractions with calmodulin activity contained a protein the electrophoretic migration of which on sodium dodecyl sulfate-polyacrylamide gel was accelerated by Ca2+. It's apparent molecular weight was about 18 or 20K in the presence or absence of Ca2+, respectively. With 45Ca-autoradiography, the protein was shown to have a high affinity for Ca2+. Thus calmodulin could be readily identified in new-born rat calvaria.