Effects of N-acetylcysteine on oxidative stress in rats with non-alcoholic steatohepatitis
Samuhasaneeto, S.; Thong-Ngam, D.; Kulaputana, O.; Patumraj, S.; Klaikeaw, N.
Journal of the Medical Association of Thailand 90(4): 788-797
2007
ISSN/ISBN: 0125-2208 PMID: 17487136 Document Number: 2843
Objective: Prove the attenuated effects of N-acetylcysteine (NAC) on oxidative stress in rats with nonalcoholic steatohepatitis (NASH).Material and Method. Male Sprague-Dawley rats were randomly divided into five groups. Group I (normal control) was fed regular dry rat chow (RC) for 6 weeks. Group 2 (NASH) wasfed 100%fat diet for 6 weeks. Group 3-5 were fed 100% fat diet for 6 weeks, and then switched to RC alone (NASH+diet; group 3), to RC+20 mg/kg/day of NAC orally (NASH+diet+NAC20; group 4) or to RC+500 mg/kg/day of NAC- orally (NASH+diet+NAC(500).; group 5) for 4 weeks, respectively. They were sacrificed to collect blood and liver samples at the end of the present study.Results: Levels of total glutathione (GSH), serum cholesterol, and hepatic malondialdehyde (MD,4) were increased significantly in the NASH group compared with normal control. Liver histopathology from group 2 showed moderate to severe macrovesicular steatosis, hepatocyte ballooning, and necroinflammation. Treatment with diet or diet plus NAC reduced the levels of GSH, cholesterol, and hepatic MDA back to normal. Liver sections from group 3-5 showed a decrease in fat deposition and necroinflammation in hepatocytes. However no differences on all variables existed between diet alone and diet plus NA C groups.Conclusion: Our data indicate that diet or diet plus NAC treatment could attenuate oxidative stress and improve liver histopathology of NASH. However the addition of NAC is not better than diet treatment alone.
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