Preparation and binding of radioactively labeled porcine transforming growth factor type beta
Ruff, E.; Rizzino, A.
Biochemical and Biophysical Research Communications 138(2): 714-719
1986
ISSN/ISBN: 0006-291X PMID: 2874805 Document Number: 283216
This report describes the labeling of porcine transforming growth factor type .beta. (TGF-.beta.) with 125-iodine. Its binding to NRK cells and three other cell lines has been examined. The data indicate that NRK cells exhibit approximately 10,000 receptors for porcine TGF-.beta. per cell, with an apparent dissociation constant of 45 pM. The binding of porcine 125I-TGF-.beta. can be blocked by porcine, murine and human TGF-.beta. but not by several well characterized growth factors. In all respects examined, the binding observed with porcine 125I-TGF-.beta. appears to be the same as that observed with human TGF-.beta. The findings reported here argue that porcine 125I-TGF-.beta. can be used to quantitate TGF-.beta. receptors on a wide range of mammalian cells.