Interference of polyester inflammation with the development of the Yoshida ascites hepatoma in the rat. II. Changes in the injected neoplastic cell volume
Ventrelli, I.; Melandri, P.; Torboli, M.; Berti, G.
Bollettino della Societa Italiana di Biologia Sperimentale 62(2): 285-291
1986
ISSN/ISBN: 0037-8771 PMID: 3718743 Document Number: 282788
The interference between the chronic inflammation caused by minced polyester threads introduced into peritoneal cavity of rats and the growth of Yoshida ascite hepatoma - previously shown - has now been confirmed and the volume changes of the cells have been studied. Rats were treated intraperitoneally with Mersilene, and injected with hepatoma immediately (TM 0) or 7 (TM 7) or 14 (TM 14) days later; rats untreated with Mersilene (NT) and injected with hepatoma were used as controls; the cells were obtained by washing the peritoneal cavity with PBS 6, 9, 18, 24, 32 and 48 hours after the inoculum. The volume of the cells was determined utilizing the Coulter Counter coupled with a pulse height analyzer. The volumetric analysis of the whole population showed a distinctly bimodal distribution, due to the presence of two cellular types picked up into a first peak (P I) with a mean volume of 100 fL, and a second peak (P II) with a mean volume of 400 fL. With NT rats, P II increased with time, apparently because of the increase in number of the neoplastic cells - which was accompanied by a certain increase in their mean volume-during the first two days after the inoculum; this increase was very small in TM 0 group, while in TM 7 and in TM 14 groups the mean volume somewhat decreased and the second peak itself disappeared in a few hours.