Short-term observation on the behavior of intrarectally instilled chenodeoxycholic acid, lithocholic acid and 5 beta-chol-3-en-24-oic acid in rats
Sawada, K.; Kawaura, A.; Tanida, N.; Shimoyama, T.
Tokushima Journal of Experimental Medicine 33(3-4): 29-37
1986
ISSN/ISBN: 0040-8875 PMID: 3448792 Document Number: 282170
Behavior of intrarectally instilled 14C-24 labeled chenodeoxycholic acid, lithocholic acid and 5.beta.-chol-3-en-24-oic acid was studied in female Fischer rats. Five rats were sacrificed hourly for 8 hours after the intrarectal instillation of 0.5 ml peanut oil solutions containing 0.5 .mu.mol bile acids made up with the respective labeled and unlabeled compounds. Radioactivities of the liver, jejunum, ileum, cecum, and proximal and distal large intestine, and their intestinal contents were measured. Conjugation of these radioactivities was analyzed by fractionation into unconjugates, taurine conjugates and sulfates on a lipophilic anion exchanger gel. Total radioactivities of the organs and intestinal content showed plateau curves which indicated that about 45, 65 and 75% of instilled chenodeoxycholic acid, lithocholic acid and 5.beta.-chol-3-en-24-oic acid were absorbed. Taurine conjugates were major constituents in the liver and small intestine throughout the experiment in all compounds, whereas unconjugates increased in the cecum and large intestine. These result indicated that the absorption of bile acids from the rectum was in accord with the general rule of passive absorption of bile acids from the intestines and that the absorption of less polar bile acids was not restrained by the unstirred water layer when they were given as oil solution.