Monoclonal antibody against bacterial lipopolysaccharide cross-reacts with DNA-histone
Sumazaki, R.; Fujita, T.; Kabashima, T.; Nishikaku, F.; Koyama, A.; Shibasaki, M.; Takita, H.
Clinical and Experimental Immunology 66(1): 103-110
1986
ISSN/ISBN: 0009-9104 PMID: 3542315 Document Number: 278974
Monoclonal antibodies to bacterial lipopolysaccharide (LPS) were prepared by fusing spleen cells from BALB/c mice immunized with Salmonella Minnesota Re 595 LPS to the mouse myeloma cell line P3U1. One of them, designated RS01, revealed a strong positive antinuclear activity and reacted with DNA-histone. RS01 also bound specifically to Salmonella Minnesota Re 595 LPS and eliminated the biological activity of LPS. The Salmonella completely inhibited the ANA activity of RS01 and DNA-histone blocked the reactivity of RS01 with LPS. Thus, it is clear that an anti-LPS monoclonal antibody, RS01 cross-reacts with DNA-histone.