Diacetylchitin coated resin hemoperfusion in dog acute pentobarbital intoxication
Wu, Z.G.; Cai, Z.H.; Lu, Z.N.; Yan, J.; Xu, R.N.
Chinese Medical Journal 99(11): 924-926
1986
ISSN/ISBN: 0366-6999 PMID: 3107934 Document Number: 278358
This paper reports the use of a diacetylchitin coated domestic macroreticular resin (surface area 600 M2/g, average porosity 85 .ANG.) as an adsorbent in artificial liver support. Chitin was extracted from the shells of small crustaceans (crabs and shrimps) and diacetylized prior to use as a coating material. 16 dogs acutely intoxicated with pentobarbital were hemoperfused either with the coated resin (n = 8) or raw resin (n = 8). The results showed that coating greatly improved resin hemocompatibility. After 125 minutes of perfusion, where was a decrease in platelets and leucocytes from 65% to 16% and 12%. Clearance rates for the coated and raw resins at a flow rate of 200 ml/min for 5 min were 195 .+-. 5 ml/min and 195 .+-. 6 ml/min. At the end of perfusion, these values were 159 .+-. 30 ml/min and 156 .+-. 16 ml/min. Diacetylchitin seems to be a promising coating material as it has good hemocompatibility without adversely affecting adsorption.