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Leukocyte content of erythrocyte concentrates without buffy coat

Mädler, J.

Zeitschrift für die Gesamte Innere Medizin und ihre Grenzgebiete 41(13): 372-374

1986

ISSN/ISBN: 0044-2542
PMID: 3765735
Document Number: 278144
In the blood constituent preparation human erythrocyte concentrate--produced from ACD-AG-blood by single buffy coat separation--the leukocyte content was conductometrically determined on the automation for haematological analyses PHA-1/2. In the erythrocyte concentrate without buffy coat (age of the initial conserved blood 8 hours) we proved an average reduction of leukocytes of 41%. The registrable whole elimination of leukocytes correlates in direct proportion with the pre-storage time of the ACD-AG bloods. Investigations of the destruction of leukocytes in the ACD-AG-blood confirmed the dependence of the leukocyte destruction on the pre-storage time, i.e., the formation of cell fragments with a volume of 60 fl, which were not seized with the method used. The 40 per cent elimination must be regarded as real size of the leukocyte reduction by single buffy coat separation.

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