Top Abstract Purchase Related
Home
>
Node
>
Subnode

Testosterone metabolism in rat liver cytosolic androgen binding assays

Sunahara, G.I.; Bellward, G.D.

Drug Metabolism and Disposition the Biological Fate of Chemicals 14(3): 366-369

1986

ISSN/ISBN: 0090-9556
PMID: 2872040
Document Number: 275731
Testosterone metabolism was studied in vitro, using incubation conditions similar to those employed in some adult male rat liver cytosolic androgen receptor assays. Following extraction, thin layer chromatography, and gas chromatography-mass spectrometric analysis, it was observed that testosterone was significantly metabolized in vitro to 5.beta.-androstane-3.alpha.,17.beta.-diol. These results indicate that gross inaccuracies can be made when estimating the free testosterone concentrations in hepatic cytosolic androgen binding studies.

Document emailed within 1 workday
Secure & encrypted payments

Waheed, A.; Winters, S.J.; Farrow, G.M.; Oshima, H.; Troen, P. 1985: Studies of the human testis. XIX. Preparation of an antibody to human testosterone-oestradiol-binding globulin and its application to the study of testicular androgen-binding protein Acta Endocrinologica 108(2): 284-288
Tremblay, R.R.; Garrido, M.; Rioux, J.E. 1973: Androgen metabolism. 1. Effect of dexamethasone on the level of testosterone production in hirsutism L'Union Medicale du Canada 102(7): 1476-1479
Krieg, M.; Bartsch, W.; Herzer, S.; Becker, H.; Voigt, K.D. 1977: Quantification of androgen binding, androgen tissue levels, and sex hormone-binding globulin in prostate, muscle and plasma of patients with benign prostatic hypertrophy Acta Endocrinologica 86(1): 200-215
Chinta, G.; Ramya Chandar Charles, M.; Klopčič, I.; Sollner Dolenc, M.; Periyasamy, L.; Selvaraj Coumar, M. 2015: In Silico and in Vitro Investigation of the Piperine's Male Contraceptive Effect: Docking and Molecular Dynamics Simulation Studies in Androgen-Binding Protein and Androgen Receptor Planta Medica 81(10): 804-812
Dirscherl, W.; Knauer, E. 1972: Effect of the antiandrogen cyproteron on testosterone metabolism in liver and seminal vesicles of the rat in vivo Arzneimittel-Forschung 22(2): 446-448
Barats, S.S.; Iordanidi, S.A. 1973: Androgen excretion in men in angina pectoris and cardialgia and changes in lipoprotein metabolism and in the blood coagulation system in androgen deficiency Kardiologiia 13(8): 74-77
Boczkowski, K.; Ozieblo, L. 1976: Testosterone concentrations and testosterone transporting protein binding capacity in blood plasma samples from patients with gonadal dysgenesis Ginekologia Polska 47(2): 191-194
Swinkels, L.M.; van Hoof, H.J.; Ross, H.A.; Smals, A.G.; Benraad, T.J. 1991: Concentrations of salivary testosterone and plasma total, non-sex-hormone-binding globulin-bound, and free testosterone in normal and hirsute women during administration of dexamethasone/synthetic corticotropin Clinical Chemistry 37(2): 180-185
Beyer, C.; Jaffe, R.B.; Gay, V.L. 1972: Testosterone metabolism in target tissues: effects of testosterone and dihydrotestosterone injection and hypothalamic implantation on serum LH in ovariectomized rats Endocrinology 91(5): 1372-1375
Bicíková, M.; Hampl, R.; Stárka, L. 1977: Binding of synthetic anabolic steroids to testosterone-estradiol binding globulin and to rat prostate cytosol Endocrinologia Experimentalis 11(2): 85-90
Bartsch, W.; Horst H J.; Derwahl, M. 1979: Interrelationships between sex hormone binding globulin testosterone 5 alpha di hydro testosterone and estradiol in blood of pre pubertal and pubertal girls Acta Endocrinologica Suppl (225): 121
Leinonen, P.; Hammond, G.L.; Lukkarinen, O.; Vihko, R. 1979: Serum sex hormone binding globulin and testosterone binding after estradiol administration, castration, and their combination in men with prostatic carcinoma Investigative Urology 17(1): 24-27
Furtner, R.; Wiche, G. 1987: Binding specificities of purified porcine brain alpha- and beta-tubulin subunits and of microtubule-associated proteins 1 and 2 examined by electron microscopy and solid-phase binding assays European Journal of Cell Biology 45(1): 1-8
Wang, C.; Swerdloff, R.S. 2002: Should the nonaromatizable androgen dihydrotestosterone be considered as an alternative to testosterone in the treatment of the andropause? Journal of Clinical Endocrinology and Metabolism 87(4): 1462-1466
Soloway, M.S. 1990: The importance of pretreatment testosterone and other prognostic variables in the response to androgen deprivation therapy Progress in Clinical and Biological Research 350: 141-148
Korneev, I.A. 2012: Practical aspects of use of hormone replacement therapy with testosterone for age-related androgen deficiency in men Urologiia 5: 122-124; 126-127
Raynor, M.C.; Carson, C.C.; Pearson, M.D.; Nix, J.W. 2007: Androgen deficiency in the aging male: a guide to diagnosis and testosterone replacement therapy Canadian Journal of Urology 14(Suppl 1): 63-68
Barbato, R.; Menabò, R.; Dainese, P.; Carafoli, E.; Schiaffino, S.; Di Lisa, F. 1996: Binding of cytosolic proteins to myofibrils in ischemic rat hearts Circulation Research 78(5): 821-828
Witherspoon, L.R.; el Shami, A.S.; Shuler, S.E.; Neely, H.; Sonnemaker, R.; Gilbert, S.S.; Alyea, K. 1988: Chemically blocked analog assays for free thyronines. I. the effect of chemical blockers on T4 analog and T4 binding by albumin and by thyroxin-binding globulin Clinical Chemistry 34(1): 9-16
Bornman, M.S.; Reif, S. 2007: Serum testosterone levels in South African men and the onset of androgen decline in ageing males South African Journal of Surgery. Suid-Afrikaanse Tydskrif Vir Chirurgie 45(2): 6264