Top Abstract Purchase Related
Home
>
Node
>
Subnode

Complement-dependent cytotoxicity of monoclonal antibodies against islet cells

Witt, S.; Hehmke, B.; Dietz, H.; Ziegler, B.; Hildmann, W.; Ziegler, M.

Biomedica Biochimica Acta 44(1): 117-121

1985

ISSN/ISBN: 0232-766X
PMID: 3888203
Document Number: 267139
Mouse monoclonal antibodies producing cell lines were generated against rat and human islet cells by somatic cell hybridization using the mouse myeloma cell line P3-X63-Ag8. Four hybrid cell lines are growing as ascites bearing tumors. All four monoclonal antibodies reacted with cytoplasmic islet cell antigens as detected by the indirect immunofluorescence technique on Bouin-fixed pancreatic sections of Wistar rats. Besides their binding to cytoplasmic antigens three of them also recognized antigens expressed at the islet cell surface. Moreover, two of these three monoclonal antibodies were capable of mediating complement dependent cytotoxicity against rat islet cells as revealed in a 51Cr-release assay.

Document emailed within 1 workday
Secure & encrypted payments

Woodhouse, C.S.; Morgan, A.C. 1989: Murine monoclonal IgG3 antibodies to human colorectal tumor-associated antigens: production and characterization of antibodies active in both antibody-dependent cellular cytotoxicity and complement-mediated cytolysis Cancer Research 49(10): 2766-2772
Ng, A.K.; Indiveri, F.; Pellegrino, M.A.; Molinaro, G.A.; Quaranta, V.; Ferrone, S. 1980: Natural cytotoxicity and antibody-dependent cellular cytotoxicity of human lymphocytes depleted of HLA-DR bearing cells with monoclonal HLA-DR antibodies Journal of Immunology 124(5): 2336-2340
Michaelis, D.; Rjasanowski, I.; Hehmke, B.; Kohnert, K.D.; Hildmann, W.; Keilacker, H.; Richter, K.V. 1985: Lack of strong association between residual human C-peptide secretion and islet cell antibodies, complement-dependent antibody-mediated cytotoxicity, and HLA antigens in newly diagnosed type I diabetics Biomedica Biochimica Acta 44(1): 99-106
Christiaans, M.H.; Nieman, F.; van Hooff, J.P.; van den Berg-Loonen, E.M. 2000: Detection of HLA class I and II antibodies by ELISA and complement-dependent cytotoxicity before and after transplantation Transplantation 69(5): 917-927
Woods, R.D.; Wesley, R.D.; Kapke, P.A. 1987: Complement-dependent neutralization of transmissible gastroenteritis virus by monoclonal antibodies Advances in Experimental Medicine and Biology 218: 493-500
Schaapherder, A.F.; Wolvekamp, M.C.; te Bulte, M.T.; Bouwman, E.; Gooszen, H.G.; Daha, M.R. 1996: Porcine islet cells of Langerhans are destroyed by human complement and not by antibody-dependent cell-mediated mechanisms Transplantation 62(1): 29-33
Falkenburg, J.H.; van der Vaart-Duinkerken, N.; Veenhof, W.F.; Goselink, H.M.; van Eeden, G.; Parlevliet, J.; Jansen, J. 1984: Complement-dependent cytotoxicity in the analysis of antigenic determinants on human hematopoietic progenitor cells with HLA-DR as a model Experimental Hematology 12(11): 817-821
Tze, W.J.; Cheung, S.S.; Tai, J. 1992: Development of monoclonal and polyclonal anti-pancreatic cell antibodies for purification and identification of porcine islet cells Transplantation Proceedings 24(6): 2837-2839
Tamerius, J.D.; Hellström, I. 1974: In vitro demonstration of complement-dependent cytotoxic antibodies to Moloney sarcoma cells Journal of Immunology 112(6): 1987-1996
Lin, Y.; Sobis, H.; Vandeputte, M.; Waer, M. 1995: Natural killer cells, antibody-dependent cellular cytotoxicity, and complement synthesis by the xenograft itself play a role in xenograft rejection Transplantation Proceedings 27(1): 286-287
Bonifacio, E.; Lampasona, V.; Genovese, S.; Ferrari, M.; Bosi, E. 1995: Identification of protein tyrosine phosphatase-like IA2 (islet cell antigen 512) as the insulin-dependent diabetes-related 37/40K autoantigen and a target of islet-cell antibodies Journal of Immunology 155(11): 5419-5426
Huber, C.; Pfister, R.; Stingl, G. 1982: Complement-dependent lymphocytotoxic antibodies (CLA) in systemic lupus erythematosus preferentially inhibit the generation of alloreactive cytotoxic T cells in secondary CML Clinical and Experimental Immunology 50(3): 525-533
Grant, C.K.; Essex, M.; Pedersen, N.C.; Hardy, W.D.; Stephenson, J.R.; Cotter, S.M.; Theilen, G.H. 1978: Lysis of feline lymphoma cells by complement-dependent antibodies in feline leukemia virus contact cats. Correlation of lysis and antibodies to feline oncornavirus-associated cell membrane antigen Journal of the National Cancer Institute 60(1): 161-166
Maruyama, S.; Sugiura, M.; Tomiyama, H.; Aizawa, T.; Ishido, T.; Kinjyo, T.; Kuwae, M.; Mimura, G. 1984: Detection of human circulating islet cell surface antibodies by dispersed rat pancreatic islet cells (III). Examination of clinical significance Rinsho Byori. Japanese Journal of Clinical Pathology 32(3): 291-298
Skopinskaia, S.N.; Tamulevich, I.A.; Iarkov, S.P.; Zlobin, V.N.; Kalinin, I.T. 1993: A highly sensitive, homogeneous method for determining antibodies and antigens by using liposomes, monoclonal antibodies and complement Zhurnal Mikrobiologii Epidemiologii i Immunobiologii 1993(1): 77-82
Parrillo, J.E.; Fauci, A.S. 1978: Comparison of the effector cells in human spontaneous cellular cytotoxicity and antibody-dependent cellular cytotoxicity: differential sensitivity of effector cells to in vivo and in vitro corticosteroids Scandinavian Journal of Immunology 8(2): 99-107
Olinescu, A.; Hristescu, S. 1984: The human effector cells of natural cell mediated cytotoxicity (NCMC) and antibody dependent cellular cytotoxicity (ADCC) express a differentiate susceptibility to pronase Archives Roumaines de Pathologie Experimentales et de Microbiologie 43(2): 147-157
Iida, S.; Nozawa, S. 1990: The mechanism of cytotoxicity of monoclonal antibodies to gynecological cancer cell-lines Nihon Sanka Fujinka Gakkai Zasshi 42(7): 667-674
Yamaguchi, Y.; Yokota, A.; Nakanishi, T.; Ueda, Y.; Takahashi, A.; Kuriya, N.; Mori, T.; Toyama, K.; Akazawa, S.; Miyake, S. 1987: In vitro production of islet cell antibodies from peripheral blood lymphocytes in insulin-dependent diabetics Journal of the Medical Association of Thailand 70(Suppl): 114-118
Scornik, J.C.; LeFor, W.M. 1987: Antibodies to crossreactive HLA antigens. Evaluation by cytotoxicity, flow cytometry, and inhibition of monoclonal antibody binding Transplantation 43(2): 235-240