Sinusoids in human cirrhotic nodules: better identification with the perfusion fixation technique
Lamouliatte, H.; Dubroca, J.; Quinton, A.; Balabaud, C.; Bioulac-Sage, P.
Journal of Submicroscopic Cytology 17(2): 279-286
1985
ISSN/ISBN: 0022-4782 PMID: 3999188 Document Number: 260427
Liver biopsies of two alcoholic patients with nodular cirrhosis were fixed by perfusion. Sinusoids, sinusoidal cells and the Disse space were well identified in the nodules. Compared to a 'normal' liver biopsy, the following abnormalities were found: a larger Disse space containing fibrillar material and numerous collagen bundles, the extension of the lateral recesses between hepatocytes, the presence of a basement membrane-like material beneath thick and irregular endothelial cells, the transformation of perisinusoidal cells into transitional cells and myofibroblasts-like cells. In addition perisinusoidal processes were clearly identified; they were thick, often containing numerous filaments, and displayed in two or more layers underlined by a discontinuous basement membrane-like material.