PCR analysis of Yq microdeletions in infertile males, a study from South India
Babu, S.R.; Swarna, M.; Padmavathi, P.; Reddy, P.P.
Asian Journal of Andrology 4(4): 265-268
2002
ISSN/ISBN: 1008-682X PMID: 12508126 Document Number: 259309
To estimate the frequency of microdeletions in the long arm of Y-chromosome of 20 infertile males from South India. Polymerase chain reaction (PCR) amplification using Y-specific STS of azoospermia factor (AZF) regions i.e., SY 84 for AZFa, SY 127 for AZFb and SY 254 for AZFc. Of the 20 infertile subjects 3 (15 %), one azoospermic and two oligozoospermic, showed microdeletions in the AZF region of Y-chromosome. The frequency of deletions involving AZF region of the Y-chromosome is 15 % in azoospermic and severely oligozoospermic infertile men. PCR amplification of AZF locus is useful for the diagnosis of microdeletions in the Y-chromosome.