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A porcine-murine hybridoma that secretes porcine monoclonal antibody of defined specificity

Raybould, T.J.; Willson, P.J.; McDougall, L.J.; Watts, T.C.

American Journal of Veterinary Research 46(8): 1768-1769

1985

ISSN/ISBN: 0002-9645
PMID: 3898933
Document Number: 257450
Spleen cells from a pig hyperimmunized with Escherichia coli were fused with nonproducer mouse plasmacytoma cells. Stable hybridoma lines secreting porcine immunoglobulins were obtained. One line secreted monoclonal porcine immunoglobulin G, which reacted specifically with E coli in an enzyme-linked immunosorbent assay and precipitated a polypeptide of molecular weight of 50,000. This is the first report of a stable porcine-murine hybridoma producing porcine antibody of defined specificity.

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