Rapid method for evaluating intra-erythrocyte Na+ activity
Antonicelli, R.; Coppa, G.; Piani, M.; Testa, I.; Russo, P.
Bollettino della Societa Italiana di Biologia Sperimentale 61(1): 151-158
1985
ISSN/ISBN: 0037-8771 PMID: 3977995 Document Number: 257292
The measurements of intracellular "Na+ activity" was performed in 10 ml of heparinized venous blood. First the blood was three times washed in isotonic magnesium chloride solution (114 mmol/l). Thereby the buffy coat was removed. Then the microhematrocrit was taken for packet cell volume determination. After the erythrocytes were lysed by ultrasound. Sodium "Na+ Activity" is measured in the hemolysate by Ion-Selective electrode. With this method all "pipetting" operations are eliminated and for the "Na+ activity" determination was used ion-selective electrode with an indirect measurements, which is less influenced by the matrix. Reference intervals determined for a healthy population were 7.3 +/- 0.6 mmol/l.