Top Abstract Purchase Related
Home
>
Node
>
Subnode

Selective cytotoxicity of an antibody-ricin a chain conjugate for human tumor cells

Tonevitskiĭ, A.G.

Molekuliarnaia Biologiia 19(4): 1034-1041

1985

ISSN/ISBN: 0026-8984
PMID: 4047033
Document Number: 257112
The toxic subunit of a plant ricin has been conjugate by a disulfide bond to a polyclonal rabbit antibody specific for the L-chain of human IgG. Both the antibody and ricin A-chain retained their original biological activity after conjugation. This conjugate proved to be a potent cytotoxin for surface Ig positive Burkitt lymphoma EB-3 cells, growing in vitro and produced 50% inhibition of protein synthesis at level of 1.4 x 10(-9) M. When tested for cytotoxic action on target cells, the composite conjugate molecule was at least 100 times more effective than antibodies alone, ricin A-chain alone or a conjugate ricin A-chain--normal rabbit IgG.

Document emailed within 1 workday
Secure & encrypted payments

Ghetie, M.A.; Moraru, I.; Margineanu, M.; Ghetie, V. 1988: Protein A vectorized toxins--II. Preparation and "in vitro" cytotoxic effect of protein A-ricin A chain conjugate on antibody coated human tumour cells Molecular Immunology 25(5): 473-477
Calafat, J.; Molthoff, C.; Janssen, H.; Hilkens, J. 1988: Endocytosis and intracellular routing of an antibody-ricin a chain conjugate Cancer Research 48(13): 3822-3827
Fulton, R.J.; Uhr, J.W.; Vitetta, E.S. 1986: The effect of antibody valency and lysosomotropic amines on the synergy between ricin a chain- and ricin B chain-containing immunotoxins Journal of Immunology 136(8): 3103-3109
DeShambo, R.M.; Krolick, K.A. 1986: Selective in vitro inhibition of an antibody response to purified acetylcholine receptor by using anti-idiotypic antibodies coupled to the a chain of ricin Journal of Immunology 137(10): 3135-3139
Woo, D.V.; Li, D.; Mattis, J.A.; Steplewski, Z. 1989: Selective chromosomal damage and cytotoxicity of 125I-labeled monoclonal antibody 17-1a in human cancer cells Cancer Research 49(11): 2952-2958
Madan, S.; Ghosh, P.C. 1992: Enhancing potency of liposomal monensin on ricin cytotoxicity in mouse macrophage tumor cells Biochemistry International 28(2): 287-295
Leonard, J.E.; Wang, Q.C.; Kaplan, N.O.; Royston, I. 1985: Kinetics of protein synthesis inactivation in human T-lymphocytes by selective monoclonal antibody-ricin conjugates Cancer Research 45(11 Part 1): 5263-5269
Bradley, T.P.; Bonavida, B. 1982: Mechanism of cell-mediated cytotoxicity at the single cell level. IV. Natural killing and antibody-dependent cellular cytotoxicity can be mediated by the same human effector cell as determined by the two-target conjugate assay Journal of Immunology 129(5): 2260-2265
Ng, A.K.; Indiveri, F.; Pellegrino, M.A.; Molinaro, G.A.; Quaranta, V.; Ferrone, S. 1980: Natural cytotoxicity and antibody-dependent cellular cytotoxicity of human lymphocytes depleted of HLA-DR bearing cells with monoclonal HLA-DR antibodies Journal of Immunology 124(5): 2336-2340
Seon, B.K. 1984: Specific killing of human T-leukemia cells by immunotoxins prepared with ricin a chain and monoclonal anti-human T-cell leukemia antibodies Cancer Research 44(1): 259-264
Olinescu, A.; Hristescu, S. 1984: The human effector cells of natural cell mediated cytotoxicity (NCMC) and antibody dependent cellular cytotoxicity (ADCC) express a differentiate susceptibility to pronase Archives Roumaines de Pathologie Experimentales et de Microbiologie 43(2): 147-157
Bright, R.K.; Shearer, M.H.; Kennedy, R.C. 1994: Immunization of BALB/c mice with recombinant simian virus 40 large tumor antigen induces antibody-dependent cell-mediated cytotoxicity against simian virus 40-transformed cells. An antibody-based mechanism for tumor immunity Journal of Immunology 153(5): 2064-2071
May, R.D.; Finkelman, F.D.; Wheeler, H.T.; Uhr, J.W.; Vitetta, E.S. 1990: Evaluation of ricin a chain-containing immunotoxins directed against different epitopes on the delta-chain of cell surface-associated IgD on murine B cells Journal of Immunology 144(9): 3637-3642
Parrillo, J.E.; Fauci, A.S. 1978: Comparison of the effector cells in human spontaneous cellular cytotoxicity and antibody-dependent cellular cytotoxicity: differential sensitivity of effector cells to in vivo and in vitro corticosteroids Scandinavian Journal of Immunology 8(2): 99-107
Thomasset, N.; Chabanas, A.; Tournaire, R.; Malley, S.; Navarro, C.; Lawrence, J.J.; Doré, J.F.; Vila, J. 1993: Selective cytotoxicity of L-glutamic acid gamma-monohydroxamate (GAH) for melanoma tumor cells Anticancer Research 13(5a): 1393-1398
Ghetie, M.A.; May, R.D.; Till, M.; Uhr, J.W.; Ghetie, V.; Knowles, P.P.; Relf, M.; Brown, A.; Wallace, P.M.; Janossy, G. 1988: Evaluation of ricin A chain-containing immunotoxins directed against CD19 and CD22 antigens on normal and malignant human B-cells as potential reagents for in vivo therapy Cancer Research 48(9): 2610-2617
Paprocka, M.; Wiedłocha, A.; Walzel, H.; Radzikowski, C. 1992: The activity of two immunotoxins composed of monoclonal antibody MoAb-16 and A-chain of ricin (MoAb-16-RTA) or A-chain of mistletoe lectin I (MoAb-16-MLIA) Archivum Immunologiae et Therapiae Experimentalis 40(3-4): 223-227
Harkonen, S.; Stoudemire, J.; Mischak, R.; Spitler, L.E.; Lopez, H.; Scannon, P. 1987: Toxicity and immunogenicity of monoclonal antimelanoma antibody-ricin a chain immunotoxin in rats Cancer Research 47(5): 1377-1382
Giardina, S.L.; Anderson, S.K.; Sayers, T.J.; Chambers, W.H.; Palumbo, G.A.; Young, H.A.; Ortaldo, J.R. 1995: Selective loss of NK cytotoxicity in antisense NK-TR1 rat LGL cell lines. Abrogation of antibody-independent tumor and virus-infected target cell killing Journal of Immunology 154(1): 80-87
Spitler, L.E.; del Rio, M.; Khentigan, A.; Wedel, N.I.; Brophy, N.A.; Miller, L.L.; Harkonen, W.S.; Rosendorf, L.L.; Lee, H.M.; Mischak, R.P. 1987: Therapy of patients with malignant melanoma using a monoclonal antimelanoma antibody-ricin a chain immunotoxin Cancer Research 47(6): 1717-1723