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An immunohistochemical staining of epidermal Langerhans' cells in tinea cruris

Emtestam, L.; Kaaman, T.; Hovmark, A.; Asbrink, E.

Acta Dermato-Venereologica 65(3): 240-243

1985

ISSN/ISBN: 0001-5555
PMID: 2411081
Document Number: 256053
Epidermal Langerhans' cells (LC) were investigated in fresh cryostat sections of 10 biopsies from 8 patients with mycologically proven tinea cruris (Epidermophyton floccosum (6), Trichophyton rubrum (2)), using OKT6 monoclonal antibodies and avidin-biotin-immunoperoxidase. Compared with the controls more epidermal LC and an increased number of LC in the upper half of the epidermis were found in the sections from tinea patients. In a double-staining method for both OKT6-positivity and hyphae, a tendency towards a gathering of LC and fungal elements was found. It is concluded that the results are in agreement with the theory that epidermal LC are responsible for the antigen uptake in dermatophytosis.

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