Changes in enzyme activities recognized in lymphocytes from patients with carcinomas of the gastrointestinal tract
Taniguchi, T.; Kiyohashi, A.; Kitsuta, T.; Okabe, H.
Cellular and Molecular Biology 31(4): 259-264
1985
ISSN/ISBN: 0145-5680 PMID: 3161615 Document Number: 251281
Changes in the activities of enzymes such as ATPase, acid phosphatase and .beta.-glucuronidase in lymphocytes from patients with carcinomas of the gastrointestinal tract (GI carcinoma) in different performance status and different immunological status were investigated in order to evaluate whether or not the changes in these enzyme activities correlate to the immune responsiveness of carcinoma-bearing patients. Patients (100) with GI carcinomas were selected as subjects and 50 normal individuals were selected as controls. The criteria of Karnofsky's performance status were used as performance status. Mononuclear cells were separated on the standard Ficoll-Conray density gradient. After preparing the crude enzyme, the activities of ATPase, acid phosphatase and .beta.-glucuronidase were determined by a modification of Skou's method, a modification of Bessey's method and a devized modification of p-nitrophenyl .beta.-glucuronide method, respectively. The changes in the activities of enzymes such as oligomycin-sensitive ATPase, bound form of acid phosphatase and .beta.-glucuronidase in lymphocytes apparently correlated to the immunological parameters in patients with GI carcinoma. These enzyme activities in lymphocytes may be evaluated as the available parameters which reflect the immune responsiveness in patients with GI carcinoma, though the changes in these enzyme activities are not considered to be specific in patients with GI carcinoma.