Quantitative localization of polystyrene microspheres following microinjection in the avian metencephalic neural crest pathway
Davis, J.U.; Bringas, P.; Slavkin, H.C.
Journal of Craniofacial Genetics and Developmental Biology 5(1): 11-19
1985
ISSN/ISBN: 0270-4145 PMID: 3988888 Document Number: 249708
Polystyrene microspheres were microinjected into crest populations at two preotic sites in Hamburger-Hamilton stage 10 and 11 chick embryos to investigate factors modulating cephalic neural crest fate. Analyses of microsphere localization and comparisons with cephalic crest fate maps indicated the following: microspheres injected at stage 10 localized with derivatives reflecting the fate of the crest population at the injection site; microspheres injected at stage 11 exhibited minimal displacement; and localization in ectoderm was similar in embryos injected at either stage. These results suggest that microinjected microspheres can be used to investigate normal and abnormal craniofacial morphogenesis.