Isolation of cDNAs encoding human and gibbon GM-CSF
Wong, G.G.; Witek-Giannotti, J.S.; Temple, P.A.; Wilkens, K.M.; Leary, A.C.; Luxenberg, D.P.; Jones, S.S.; Brown, E.L.; Kay, R.M.; Orr, E.C.
Progress in Clinical and Biological Research 191: 351-366
1985
ISSN/ISBN: 0361-7742 PMID: 3876571 Document Number: 249465
We have used a mammalian cell expression cloning system to identify cDNA clones encoding human granulocyte-macrophage colony stimulating factor. The human clone was used as a hybridization probe to identify the corresponding sequence from a cDNA library prepared from a gibbon T-cell line. The human cDNA has been used to produce recombinant GM-CSF in monkey COS-1 cells. The purified protein from COS cells is very similar to the GM-CSF isolated from a continuous human T-cell line.