Jaffes' reaction for creatinine: kinetic study of the reactions of creatinine, glucose and creatinine and glucose with alkaline picrate
Mali, B.; Nicholas, P.C.
Biochemical Society Transactions 16(3): 395-396
1988
ISSN/ISBN: 0300-5127 DOI: 10.1042/bst0160395Document Number: 249102
The study of Jaffes' reaction for creatinine is extended to investigate the simultaneous spectrophotometric kinetic aspects of the reactions that occur between creatinine, glucose, and glucose and creatinine and alkaline picrate. It is concluded that the rates of reaction of the creatinine and glucose reactions are additive and, within the limits of experimental error, are equal to the rate of reaction of the corresponding glucose-creatinine reaction. Glucose interferes with the estimation of creatinine.