A radioimmunological technic for the assay of human albumin at low concentrations
Mariani, G.; Luti, R.
Quaderni Sclavo di Diagnostica Clinica E di Laboratorio 21(2): 144-153
1985
ISSN/ISBN: 0033-4979 PMID: 3878532 Document Number: 248059
In the present study a radioimmunoassay procedure for the measurement of human albumin is described which presents some modifications with respect to an earlier method used for the measurement of albumin in peritoneal dialyzate. The results of electrolytic labelling of albumin, the kinetic curve on the antigen-antibody reaction and the cross reactivity of the anti-albumin antiserum with alpha 1 antitrypsin are reported. The typical standard curve obtained with the proposed method allows the estimation of human albumin in biological fluids where the protein is present at very low quantities, not measurable by traditional complexometric and colorimetric techniques.