Deletion of late genes of the prophage lambda
Matvienko, N.I.; Troianovskaia, I.N.; Zheleznaia, L.A.; Iarchuk, O.B.
Molekuliarnaia Biologiia 19(6): 1457-1465
1985
ISSN/ISBN: 0026-8984 PMID: 3001508 Document Number: 244975
The deletions in tandem prophage lambda appear with high frequency (to 10%) in rec A- strain of Escherichia coli. The deletions were shown by marker rescue and hybridization of fragments of DNA on nitrocellulose filters with nick-translated phage lambda DNA localized only in prophage area. Right and left att sites are not involved. The majority of defective lysogens had all regulatory regions and deletions of late structural genes. These strains may be used for construction of the host-vector systems with the strongest promoter p'R of phage lambda.