Fetal intrauterine death and fetal malformation induced by trypan blue in rats
Ema, M.; Itami, T.; Kawasaki, H.; Kanoh, S.
Eisei Shikenjo Hokoku. Bulletin of National Institute of Hygienic Sciences 1985(103): 101-103
1985
ISSN/ISBN: 0077-4715 PMID: 3830288 Document Number: 242484
The relationship between fetal intrauterine death and fetal malformation was studied in Wistar rats. Pregnant rats that had received trypan blue subcutaneously at a dose of 250 mg/kg on day 7 of pregnancy were killed on day 12, 14, 16 or 20 of pregnancy, and the fetuses were examined. The rate of the fetal intrauterine deaths increased with the increase in days after the administration of trypan blue. Conversely, the incidence of fetuses with external malformations decreased as pregnancy continued. The same decrease was observed in the incidences of fetuses with some particular malformations such as anomaly of tail and exencephaly. It was suggested that some of the fetal intrauterine deaths resulting from the administration of trypan blue were secondary to fetal malformation.