The use of fluorescence probes in the study of the beta-adrenoreceptor function of human erythrocytes
Sominskiĭ, V.N.; Bluma, R.K.; Kalninia, I.E.
Biofizika 30(4): 642-645
1985
ISSN/ISBN: 0006-3029 PMID: 2996618 Document Number: 242382
Possibility of making use of fluorescent probe 4-(p-dimethyl aminostyril)-1-methyl pyridinium p-toluene-sulphonate (DSM) for observing the interaction of beta-adrenoactive substances (adrenaline and propranolole) and specific receptors of human blood erythrocytes has been studied. Two types of DSM bindings with erythrocyte membranes have been revealed--specific and non-specific. It has been found that DSM reacts selectively to preliminary influence of beta-adrenoactive substances: antagonist propranolole diminishes both specific and nonspecific bindings and agonist adrenaline increases specific binding with the membrane erythrocytes. The conclusion is made as to possible use of DSM probe for investigating beta-adrenoreceptive function of the cell membranes.