Derivatives of benzo (c) fluorene. XI. Effect of Benfluron on hemopoietic stem cells
Hrubá, A.; Paluska, E.; Pujman, V.; Polednová, V.; Chudomel, V.; Krepelka, J.
Neoplasma 31(4): 407-413
1984
ISSN/ISBN: 0028-2685 PMID: 6472513 Document Number: 241911
The effect of the recently produced cytostatic drug Benfluron - 5-(2-N,N-dimethylamino-ethoxy)-7-oxo-7H-benzo(c)fluorene hydrochloride - on hemopoietic stem cells was tested by the production of spleen cell colonies (CFU-S) in irradiated mice following application of bone marrow cells and by the production of hemopoietic colonies (CFU-C) in semisolid agar. The reduced numbers of CFU-S were found in mice treated with Benfluron or Benfluron-treated bone marrow cells. The increased numbers of CFU-S were found in mice receiving bone marrow cells from Benfluron-treated donors. The production of CFU-C was decreased after 2 h incubation of bone marrow cells with 0.5-5 .mu.g/ml of Benfluron, and fully inhibited at higher concentrations.