Filipin-positive, oil red O-negative particles in atherosclerotic lesions induced by cholesterol feeding
Kruth, H.S.
Laboratory Investigation; a Journal of Technical Methods and Pathology 50(1): 87-93
1984
ISSN/ISBN: 0023-6837 PMID: 6694353 Document Number: 240506
In this study, extracellular spherical filipin-positive particles have been demonstrated using the fluorescent cholesterol probe, filipin, in rat, rabbit, and monkey atherosclerotic lesions induced by cholesterol feeding. Examination of tissue sections stained with both filipin (detects unesterified cholesterol) and oil red O (detects esterified cholesterol) demonstrated that extracellular filipin-positive particles did not stain with oil red O. Thus, extracellular filipin-positive particles represent a previously unrecognized structure in which cholesterol accumulates within atherosclerotic lesions.