Preparation and experimentation of an antidigitalin monoclonal antibody: interest in human treatment
Edelman, L.; Colignon, A.; Shermann, J.M.; Girre, C.; Dally, S.; Reviron, J.
Developments in Biological Standardization 57: 343-347
1984
ISSN/ISBN: 0301-5149 PMID: 6526144 Document Number: 236534
A monoclonal antidigitalin antibody was obtained following the immunization of BALB/mice with digitalin coupled to BSA, and the fusion of lymphocytes from these mice with murin myeloma 8653. The supernatants were screened by a radioimmunoassay in which the supernatant was incubated with digitalin coupled to I125. This monoclonal antibody "Dig 278" has an antigen binding ratio of 95%; is an IgGl; has a titre of 1/1000 and its affinity constant, as determined by a Scatchard plot, was 1,20(9) L/M. By experimenting with the monoclonal antibodies on 15 rabbits, we have obtained the reversal of heart rhythm disorders and the survival of animals injected with a lethal dose of digitalin. All the controls died. This antibody could prove useful in treating advanced cases of digitalin intoxication and for which no satisfactory treatment is available at present.