Investigation of sweat production in the isolated rat eccrine sweat gland
Michniak, B.B.
Patologia Polska 35(2): 231-238
1984
ISSN/ISBN: 0031-3114 PMID: 6514423 Document Number: 235913
Sweat production was induced using carbachol and methacholine in isolated rat plantar sweat glands. These were maintained in a suitable environment (modified Krebs-Ringer bicarbonate at 38.degree. C containing 30% w/v [wt/vol] fresh rat serum). The output of sweat was measured every 5 min for 85 min and the maximal final volume obtained with any 1 gland was 23 nl. Sweat production ceased on administration of appropriate concentrations of atropine or the withdrawal of Ca ions from the bathing fluid.