Cell-free synthesis of rat epidermal growth factor-like polypeptide

Majumdar, A.P.; Frazier, M.L.

Research Communications in Chemical Pathology and Pharmacology 46(1): 103-111

1984


ISSN/ISBN: 0034-5164
PMID: 6334338
Document Number: 230506
Poly(A+)RNA isolated from the adult male rat salivary gland has been found to direct the synthesis of immunoreactive EGF polypeptide in the wheat germ cell-free translation system. When the immunoreactive translation products were analyzed by sodium dodecyl sulfate polyacrylamide gel electrophoresis, a single radioactive peak which co-migrated with the standard 125I-EGF (mouse) was observed.

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