Determination of the nucleotide content of DNA blocks by measuring the area under the melting curve
Vardapetian, R.R.; Vardevanian, P.O.; Panosian, G.A.
Biofizika 29(3): 489-491
1984
ISSN/ISBN: 0006-3029 PMID: 6466726 Document Number: 229794
A method for determining the nucleotide content of DNA blocks from areas under the melting curve is suggested. It is shown that this method is more simple and the total melting curve is decomposed not to the Gauss components, but it is considered as as superposition of the melting curves of individual blocks. Application of the method for the determination of calf thymus DNA is discussed.
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