Central alpha 2-adrenergic receptors in the rat: effect of sodium in vitro
Parini, A.; Marchesi, E.; Fratino, P.; Zoppi, A.; Finardi, G.
Bollettino della Societa Italiana di Biologia Sperimentale 60(6): 1183-1189
1984
ISSN/ISBN: 0037-8771 PMID: 6089852 Document Number: 229484
Characteristics of the (3H) Rauwolscine binding were studied in homogenates of rat cerebral cortex, particularly in presence of NaCl. We had shown that (3H) Rauwolscine specifically bind the alpha 2-adrenoceptors. The Scatchard plot analysis had demonstrated 2 sites: a high affinity site (B 41,06 +/- 5,21 KD = 1,31 +/- 0,16) and a low affinity site (B = 203,41 +/- 13,37 KD = 11,62 +/- 0,55 nM). Incubation of cerebral cortex homogenates with NaCl 150 mM induced an increase (53,97 p less than 0.001) of the high affinity site density. These results indicate that (3H) Rauwolscine is a useful alpha 2-adrenoceptor ligand to study in animal models various central nervous system abnormalities or diseases induced by ion disorders.