Modulation of macrophage Ia-expression by lipopolysaccharide. I. Induction of Ia expression in vivo
Ziegler, H.K.; Staffileno, L.K.; Wentworth, P.
Journal of Immunology 133(4): 1825-1835
1984
ISSN/ISBN: 0022-1767 PMID: 6332135 Document Number: 228062
Experiments were performed to analyze the modulation of macrophage Ia expression and biosynthesis by Salmonella minnesota-derived lipopolysaccharide (LPS) in vivo. The i.p. injection of LPS into LPS-responder mice caused a dramatic increase in the Ia expression of the peritoneal macrophage population harvested 1 wk after injection. As little as 1 ng of lipid-rich Re595 LPS/mouse caused a significant I-Ak increase, and 1 .mu.g was optimal; wild-type S. minnesota LPS was less active. No I-Ak induction by LPS was observed in the LPS-nonresponder strain C3H/HeJ. LPS-induced macrophages showed a 6- to 16-fold increase in I-Ak expression by radioimmunoassay (RIA), a 3- to 10-fold increase in the proportion of I-Ak-positive cells and a 10- to 15-fold increase in I-Ak biosynthetic capacity. The magnitude of this induction by LPS was comparable to increases observed after injection to live Listeria monocytogenes. The kinetics of I-Ak induction by LPS and by L. monocytogenes were different; LPS caused an initial decrease in I-Ak expression 1 day after injection, and I-Ak induction by LPS occurred more slowly and maintained heightened expression longer. Several H-2 gene products (H-2Kk, I-Ak and I-Ek) were augmented in LPS-induced macrophages. In keeping with increased I-A and I-E expression, LPS-induced macrophages were more effective than normal macrophages were in presenting antigen to T lymphocytes. Modulation of macrophage Ia expression is evidently an important mechanism contributing to the immunoregulatory activity of LPS.