Immunohistochemical localization of apolipoprotein B in human atherosclerotic lesions
Yomantas, S.; Elner, V.M.; Schaffner, T.; Wissler, R.W.
Archives of Pathology and Laboratory Medicine 108(5): 374-378
1984
ISSN/ISBN: 0003-9985 PMID: 6370192 Document Number: 227544
A peroxidase-antiperoxidase immunohistochemical method was used to detect the presence of intracellular and extracellular apolipoprotein B (apo B) in paraffin sections of human abdominal aortas and coronary arteries with and without atherosclerotic involvement. The four aortas studied disclosed both intracellular and extracellular apo B in areas in which lipid was found by oil red O staining. Coronary arteries also demonstrated abundant extracellular apo B but failed to disclose intracellular apo B in cells with stainable lipid. Immunohistochemical detection of intracellular apo B in raised aortic lesions but not in cells of coronary lesions suggests that arteries that contain detectable amounts of intracellular apo B correspond to arteries that usually have earlier atherosclerotic involvement.