Methodological specifications on the Todd method and preliminary results
Doni, A.; Bartoloni Saint Omer, F.; Di Lollo, S.; Gabbrielli, G.; Leoncini, G.; Piovanelli, C.; Rosi, E.
Bollettino della Societa Italiana di Biologia Sperimentale 60(4): 731-737
1984
ISSN/ISBN: 0037-8771 PMID: 6732946 Document Number: 226070
In order to measure the potentiality and kinetics tissue fibrinolysis we have modified Todd's histochemical method preparing at least six sections taken from same sample tissue, by infraoperatory biopsy, esponing them to a fibrin film at a constant concentration; we have also chosen to incubate the section at 10, 20, 30, 45, 60 min. The measurement of the areas of fibrin, of tissue and fibrinolysis, at the above mentioned times, has been effected at standard magnification (15 X) by an image analyser (Videoplan) scale 1:8. For each sample we suggest to elaborate an Index of the Potentiality of Fibrinolysis (formula; see text) and an Index of the Kinetics of the Fibrinolysis (formula; see text). Applying this method to two different groups of thyroid disease (struma and adenoma) we have not pointed out any statistically significant difference.