Usefulness of a genotypic resistance test using dried blood spot specimens in African HIV-infected children with virological failure according to the 2010-revised WHO criteria
Charpentier, C.; Gody, J-Chrysostome.; Tisserand, P.; Matta, M.; Fournier, J.; Mbitikon, O.; Bélec, L.
Archives of Virology 156(9): 1603-1606
2011
ISSN/ISBN: 0304-8608 PMID: 21479946 DOI: 10.1007/s00705-011-0997-9Document Number: 224893
We compared paired plasma and dried blood spot (DBS) samples from 54 HIV-1-treated children living in Bangui, Central African Republic, for antiretroviral-resistance-associated mutations. All children displayed virological failure (HIV-1 RNA >3.7 log1copies/ml). Testing for resistance genotype was carried out in a reference laboratory in Paris, France. A successful test result was obtained in 54 (1%) plasmas and 25 DBSs (46%). Among the 732 resistance-associated mutations analyzed, 718 were identical, leading to a high concordance rate of 98.1%. Genotypic resistance tests on DBS samples were found to be highly feasible and accurate in a foreign reference laboratory, but with additional costs for shipping and decreased sensitivity.
Document emailed within 0-6 h
