Granulocyte activation by endotoxin. II. Role of granulocyte adherence, aggregation, and effect of cytochalasin B, and comparison with formylated chemotactic peptide-induced stimulation
Dahinden, C.; Fehr, J.
Journal of Immunology 130(2): 863-868
1983
ISSN/ISBN: 0022-1767 PMID: 6294179 Document Number: 220785
Polymorphonuclear leukocyte (PMN) stimulation by endotoxin is heavily dependent on incubation conditions: although marked increase of PMN adhesiveness, enzyme release, hexose monophosphate shunt activity and superoxide production occur when endotoxin-challenged cells are incubated in stationary petri dishes, absolutely no such response is observed with cells kept in suspension. Preincubation with cytochalasin B does not alter this reaction pattern. Hyperadhesiveness of endotoxin-challenged PMN is retained after thorough washing, eliminating the possibility that adhesion is due to absorption of endotoxin to petri dishes. In contrast to formylated chemotactic peptides that stimulate PMN adherence and aggregation, endotoxin elicits absolutely no aggregatory response. Preexposure to endotoxin does not block the aggregatory response to subsequent addition of chemotactic peptides, whereas effects of endotoxin and formylated peptides on PMN in the stationary system are additive. Surface contact is apparently an essential primary event in PMN stimulation. [Human PMN and endotoxin preparations from Salmonella abortus-equi, S. minnesota and Escherichia coli were used in these studies.].