Etiopathogenesis and sequelae of viral neuroinfections in children. I. Principles, materials and methods of study

Kańtoch, M.; Imbs, D.; Czachorowska, M.; Jankowski, M.; Jarzabek, Z.; Madaliński, K.

Medycyna Doswiadczalna i Mikrobiologia 35(3-4): 235-248


ISSN/ISBN: 0025-8601
PMID: 6328139
Document Number: 217668
The principles, materials and methods of a long-term study on viral neuroinfections in children which began in 1979 are presented. The aims of the study were as follows: determination of the role viruses play in neuroinfections in children, determination of the relationship between virus agent and clinical course of the disease and its late sequelae, evaluation of various serological and virological methods for diagnosis of viral neuroinfections in children and of the examination of the immune reactions in such infections. Clinical material was collected from patients hospitalized from Aug. 1979-July 1981. Patients (673) with viral neuroinfection, aged up to 14 yr, including 583 cases of meningitis (86.6%), 16 with simultaneous encephalitis and 6 with cranial nerve paralysis, 23 cases of encephalitis (3.4%), 48 cases of cranial nerve paralysis (7.1%) and 19 cases of other syndromes (2.8%) were studied. Virological and serological methods applied were directed toward the possible viral agents: enteroviruses, mumps virus, herpes virus, cytomegalovirus, measles virus, influenza virus, parainfluenza virus, respiratory syncytial virus and rubella virus. Clinical material was collected, depending on viral agents sought, by throat swabs and examination of serum and feces. The methods applied were tissue culture, neutralization, complement fixation, hemagglutination inhibition, immunofluorescence, enzyme-linked immunosorbent assay and determination of immune complexes and complement level (including anticomplementarity).

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