DNA repair dependent NAD+ metabolism is impaired in cells from patients with Fanconi's anemia
Klocker, H.; Auer, B.; Hirsch-Kauffmann, M.; Altmann, H.; Burtscher, H.J.; Schweiger, M.
EMBO Journal 2(3): 303-307
1983
ISSN/ISBN: 0261-4189 PMID: 11894942 Document Number: 217108
In vitro cultivated fibroblasts derived either from patients with Fanconi's anemia (FA) or from healthy probands were analyzed for their DNA repair-dependent NAD+ metabolism. No difference in NAD+ pools was found. NAD+ consumption after cell damage by u.v. irradiation was, however, significantly reduced in FA cells. Several FA cell lines had a lowered ability to transfer ADP-ribose to acid-precipitable material. Additionally, a decreased activity of NAD: protein ADP-ribosyltransferase was found for three FA cell lines. Our data indicate, that FA is accompanied by a defective NAD+ metabolism during DNA repair.