Characterization of clofibrate-stimulated alkaline phosphatase in rat liver
Popova, S.V.; Antonenkov, V.D.; Panchenko, L.F.
Voprosy Meditsinskoi Khimii 29(4): 78-83
1983
ISSN/ISBN: 0042-8809 PMID: 6623998 Document Number: 211853
Hypolipidemic agent clofibrate induced the alkaline phosphatase activity in rat liver but did not affect this activity in kidney, brain, heart and intestine. In liver cells the clofibrate induced alkaline phosphatase was localized in plasma membrane and cytosol and was similar to the enzyme found in normal hepatocytes by its main properties (pH optimum, inactivation temperature, substrate specificity, Km, effect of inhibitors and inducers). The putative mechanism of the alkaline phosphatase induction in liver tissue by means of clofibrate is discussed.