Influence of magnetic field on hydrogen peroxide decomposition by catalase
Vanag, V.K.; Kuznetsov, A.N.; Piruzian, L.A.
Biofizika 28(1): 18-23
1983
ISSN/ISBN: 0006-3029 PMID: 6830896 Document Number: 211369
The rate constants of H2O2 decomposition equal to 0.45 X 10(7) M-1S-1 per hem and Michaelis constants higher than 0.3 M were found by gas volume meter and spectrophotometer methods for purified preparations of catalase from bovine liver. Unlike the results obtained earlier the magnetic field with induction 0.65 T and 0.012 T does not affect the constant rate within 3%. It was found with the substrate concentration less than 0.01 M when the classical catalase mechanism was observed and with higher concentration of the substrate up to 0.7 M when the catalase inhibition by H2O2 played an important role.