Changes in HDL subfractions in patients with type I diabetes mellitus before and after metabolic control
Pacifico, A.; Cherchi, G.M.; Baggi, M.A.; Deiana, M.G.; Maioli, M.
Bollettino della Societa Italiana di Biologia Sperimentale 59(11): 1618-1624
1983
ISSN/ISBN: 0037-8771 PMID: 6365116 Document Number: 204632
In order to further investigate the behaviour of high density lipoproteins in diabetes mellitus, we studied HDL subclasses, HDL2 and HDL3, in 10 patients with newly detected, untreated insulin-deficient diabetes before starting insulin treatment and after getting a good metabolic control. We used the extractive method of Abell to determine HDL-cholesterol after LDL and VLDL precipitation with polyanions and HDL3-cholesterol after HDL2 precipitation with dextransulphate 15,000 m.w. After insulin therapy, we observed a significant increase in HDL-cholesterol and a decrease in serum triglycerides. Only HDL2-cholesterol, but not HDL3-cholesterol, raised; moreover, we found a significant inverse relationship between HDL-cholesterol (and also HDL2-cholesterol) and triglycerides. So, we think that an increase of lipoprotein lipase activity, owing to insulin treatment, could account for our results.