The role of serum lipoproteins on the in vitro proliferative potential of human hematopoietic progenitors CFUC and CFUE
Douay, L.; Barbu, V.; Baillou, C.; Najman, A.; Gorin, N.C.; Polonovski, J.; Duhamel, G.
Experimental Hematology 11(6): 499-505
1983
ISSN/ISBN: 0301-472X PMID: 6617786 Document Number: 202823
The influence of various lipoprotein fractions on the proliferation of normal human hematopoietic progenitors, CFUC and CFUE, was studied in vitro. The lipoprotein fractions, very low density lipoproteins (VLDL), intermediate density lipoproteins (IDL), low density lipoproteins (LDL), high density lipoproteins (HDL2 and HDL3), were isolated by sequential ultracentrifugation. The addition of each subfraction to lipoprotein deficient medium allowed us to distinguish two categories of lipoproteins: firstly, those with density d greater than 1.030, LDL, HDL2 and HDL3 which showed a marked inhibitory activity on CFUC and CFUE proliferation and, secondly, those with density d less than 1.030, VLDL and IDL which did not show any inhibitory activity. The regulatory role of lipoproteins on progenitor cell proliferation is discussed.