Possibilities for pathogenetic clarification of hemolytic anemias using incubation hemolysis
Grieger, M.; Leppin, K.
Zeitschrift für die Gesamte Innere Medizin und ihre Grenzgebiete 38(11): 301-306
1983
ISSN/ISBN: 0044-2542 PMID: 6613200 Document Number: 200595
The haemolysis is the uniform answer of red blood cells to detrimental effects. The method of incubation haemolysis was controlled for its evidence concerning certain groups of causes. The determination of the free haemoglobin in the plasma before incubation may be regarded as measure of haemolysis in vivo. Compensation of the Hb-release under addition of glucose indicates to such causes which are accompanied by an increased expenditure of energy, e.g. transports of ions, protein kinases, spectrin phosphorylations. The incubation with ATP is effective above all in such cases of a haemolysis in which by enzyme defects the red blood cell itself is not able to the sufficient syntheses of connections rich in energy. Some instances for causes of an increased membrane permeability for Hb suggest that the Dacie-test allows only a superficial orientation to groups of diseases.