Isolation of skeletal muscle plasmatic membranes as closed vesicles
Diadiusha, G.P.
Ukrainskii Biokhimicheskii Zhurnal 55(2): 194-198
1983
ISSN/ISBN: 0201-8470 PMID: 6845445 Document Number: 198287
Ultrasonic treatment of skeletal muscle sarcolemma preparations in 0.25 M sucrose, 0.1 M NaCl, 0.001 M tris-HCl-buffer (pH 7.4) induces formation of closed osmotically active vesicles which may be isolated by centrifugation of a sonicated suspension within the sucrose density gradient. The vesicles retain the enzymic activity peculiar to the initial sarcolemma preparations.
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