Determination of beta-mannanase activity by viscosimetric and spectrophotometric methods
Firantas, S.G.; Venozhinskene, I.I.; Pauliukonis, A.B.
Prikladnaia Biokhimiia i Mikrobiologiia 18(4): 581-587
1982
ISSN/ISBN: 0555-1099 PMID: 6812038 Document Number: 195459
The activity of beta-mannanase from Bacillus subtilis was measured viscosimetrically and spectrophotometrically. As substrate galactomannane of Ceratonia siliqua was used. Relationships between the beta-mannanase activity and the substrate concentration as well as the enzyme content were investigated. The kinetic parameters of the enzymes obeying the Michaelis-Menten equation were calculated. It was found viscosimetrically that Vmax of the commercial enzyme preparation was 1.4 mucat/g (at pH 5.8 and 40 degrees) and Km was 0.6 mM. The viscosimetric method shows high sensitivity, whereas the spectrophotometric technique suits mass-scale analyses.